The survey results indicate that mouse is the overwhelmingly preferred laboratory animal. Labome has surveyed formal publications citing animal models to provide an overview of animal models in publications table 1. These reports are organized on the basis of the breeding room of origin. Serology profiles mouse our primary serology testing method is the multiplexed fluorometric immunoassay, or mfia. The standard of care at ucsf is to prevent animal pain whenever possible and to treat animal pain whenever diagnosed. Medications for laboratory animals administration of any treatments must be done in consultation with a veterinarian. The divergence time between primates and rodents was assumed to be 122 million years ago mya based on the study of easteal et al. Mouse hepatitis virus mhv division of animal resources university of illinois, urbana background. Standard operating procedure the jackson laboratory. Molecular evolutionary analyses of the rh blood group. All medications delivered in food or water must be clearly identified with the treatment that is being administered.
The house mouse, mus musculus, is a complex species comprised of several subspecies. Featuring newly revised european standards on laboratory animals, this book is the uptodate authority on the care of mice in laboratory research internationally. Laboratoryreared rats and mice are specified pathogenfree and are typically accompanied by a. Purpose the intent of this standard operating procedure sop is to describe procedures for castrationsurgery in rodents. The jackson laboratory mouse colony management and breeding strategies. Malocclusion is a common disorder of many strains of laboratory mice and is readily diagnosed by an oral examination.
All books are in clear copy here, and all files are secure so dont worry about it. A few of the previously published sections sexual maturity, estrous cycle, mating, and gestation have been rewritten. To accomplish this, periodic inspections of stocks must be conducted. Chemicalwaste storage chemical storage shelves shall not be placed above laboratory sinks. Intravenous injection tail vein mouse revised may 2016 1 laboratory animal biomethodology workshop u the laboratory mouse intravenous injection tail vein 1. Substance solutions should be isotonic same tonicity as blood. Mouse hepatitis virus is probably the most important pathogen of laboratory mice. Put on a hairnet, face mask, disposable lab coat, and a pair of shoe covers. Jax mice are bred within and shipped from multiple breeding rooms. This guide describes the development of humanized mice and how. Mice are capable of detecting a wide range of auditory frequencies, and can hear both audible sounds and ultrasound frequencies. Zoonoses associated with laboratoryreared rodents this information sheet is intended for princeton university faculty, students and staff who conduct research with laboratory rodents, including mice and rats.
In mouse facilities, breeding should be reduced to the minimum. Electronic version of biology of the laboratory mouse, earl l. Standard operating procedure the jackson laboratory mouse. Laboratory mice are inbred so that each strain is homo zygous and has a unique mhc haplotype. Development of the laboratory mouse began with pet fanciers who bred mice for their unique coat colors. Infection of skin scratches and bite wounds with mouse or humanborne opportunistic microbes demands attention through a strict occupational medical treatment program national research council, 1997. Behavioral measures c57bl6 mice of both sexes given a choice between 202530 c spent the majority of their time in 30 c. All personnel working with animals, their tissues, or working in areas where animals are housed must submit an animal use medical screening aums form every 3 years to screen for exposure to possible health hazards in the work environment. Usually these animals are maintained in germfree, gnotobiotic, or specificpathogenfree facilities. Handbook on genetically standardized mice we would like to thank the handbook steering committee, whose membership has included susie airhart, carol bult, greg cox, muriel davisson, edward leiter, cathy lutz, john. Clinical signs and lesions spontaneous infections with mtv are asymptomatic. A brief history of mouse research is also offered, ranging from their first use in genetics experiments to nobel prizewinning discoveries in immunology and neurobiology. The mouse grimace scale offers a standardized behavioral coding system to study the subjective pain experience in mice.
Read online mouse genetics book pdf free download link book now. Wait for the mouse to be still with no walking or rearing. Zoonoses associated with laboratory reared rodents this information sheet is intended for princeton university faculty, students and staff who conduct research with laboratory rodents, including mice and rats. Technical specifications test menuclotting, chromogenic and immunological assays continuous sample and reagent loading. Manipulating the mouse embryo a laboratory manual fourth edition.
The mouse should have very limited movement of the head. Examples of the developmental stages of albino, agouti, and black pups are shown. They are the most commonly used mammalian research model and are used for research in genetics, psychology, medicine and other scientific disciplines. The use of controls to monitor the analytical performance and reliability of test methods is an essential component of good laboratory practice. As a result, mouse colonies everywhere are expanding, and scientists who previously focused on other models are turning their attention to the mouse. Coding of facial expressions of pain in the laboratory mouse. Relative evolutionary distances between mouse and rat, and between human and macaque were 0. Laboratory design guidelines 20 design guidelines 6 page vi. Manipulating the mouse embryo cold spring harbor laboratory. Standard operating procedures clinical and translational research center purpose.
Put the mouse down on the metal grating in the front of the hood, so the mouse can grab on to the grating. Fourcolor illustrated book with complete instruction on mouse surgery, anatomy, behavior and care of the mouse in laboratory research new chapters on new. A mouse of bc4 status was wellfleshed, and the spine was a continuous column unless palpated with firm pressure. Indepth manuals and guides for using laboratory mice in research. These mouse models are accelerating the development of novel therapies in hiv infection and oncology.
Revised to reflect advances since the first edition, the laboratory mouse, second edition continues to be the most accessible reference on the biology and care of the laboratory mouse. The use of laboratory animals, including mice, continues to be an important part of biomedical. Nevertheless, it was not until clarence cook little developed the first inbred mouse strain, dba, in 1909 that the modern laboratory mouse was born. By 1901, william castle, the greatgrandfather of mammalian genetics, recognized the potential of mice to test mendels theories. The mouse grimace scale is a standardized behavioral coding system that demonstrates facial expressions which can be used to assess pain in the laboratory mouse. Paper nesting material as cage enrichment for laboratory mice. Anatomy of the laboratory mouse pdf free download epdf. The laboratory mouse, 2e is a comprehensive book written by international experts. A laboratory manual, fourth edition click here for more information or to buy the book.
Grab the skin on the mouses back between your thumb and index finger. Working with mice and rats animal use medical screening aums. The laboratory mouse, second edition is a comprehensive book written by international experts. The mouse genome database mgd is the key knowledgebase for integrated and comprehensive access to genetics and genomics data for the laboratory mouse, with a primary goal of facilitating the use of the mouse as a model system for understanding human biology and disease 1,2.
Electronic version of biology of the laboratory mouse edited by earl l. The laboratory mouse laboratory animal pocket reference. Laboratory reared rats and mice are specified pathogenfree and are typically accompanied by a. The laboratory mouse is the most common used mammalian research model in biomedical research. Ultrasound frequencies are used by mice to communicate during sexual activities, and are also the frequencies of the distress calls of the infant mouse. This chapter is a partial revision of a chapter in biology of the laboratory mouse snell, 1941. Laboratory mice are usually of the species mus musculus. This site is like a library, you could find million book here by using search box in the header. Download mouse genetics book pdf free download link or read online here in pdf. It is imperative that we take action to reduce the spread of covid19 in our population, to flatten the curve and thereby prevent the many unnecessary.
Animal anesthesia, analgesia and pain management are crucial components of the animal use protocol. Locate the left midplantar surface of the hind paw of the mouse and identify the middle of the paw application site the jackson laboratory mouse neurobehavioral phenotyping facility jaxmnbf. Jul 30, 2012 the laboratory mouse, second edition is a comprehensive book written by international experts. University laboratory animal resources mouse analgesia. Biology of the laboratory mouse mouse genome informatics. With inclusions of the newly revised european standards on laboratory animals, this will be the most current, global authority on the care of mice in laboratory research. Give one tabletmouseday provides 2 mgkg dosing for an average size 25 g mouse direct injectable, oral gavage add 0. The laboratory mouse handbook of experimental animals. Solutions should be isotonic same tonicity as blood. The mhc haplotype in these strains is designated by a small letter a, b, d, k, q, s, etc. Additionally the laboratory mouse continues to play a widely publicized vital role in the human genome project. Anesthesia and analgesia in laboratory animals at ucsf. The oral glucose tolerance test ogtt is the gold standard test for the diagnosis of diabetes mellitus, although it is often possible to establish this diagnosis without resorting to a full ogtt. The laboratory mouse is a small mammal of the order rodentia which is bred and used for scientific research.
Mouse models the laboratory mouse is a powerful tool that scientists use to model human diseases and conditions in the search for better treatments and cures for humankinds most devastating diseases. They comprise the majority of all experimental mammals and tend to be the model of choice used for research into many diseasesdisorders including cancer, heart disease, asthma, alzheimers, down syndrome, deafness, osteoporosis, obesity, diabetes and even mental health research. Usually these animals are maintained in germfree, gnotobiotic, or. The jackson laboratory mouse neurobehavioral phenotyping facility jaxmnbf standard operating procedure page 9 of 10 document number. Swipe your chicago card university of chicago id through the reader to enter the mouse facility. These fancy mice then became subjects for research due to interest in the mechanisms of inheritance of these coat colors. Although the infection generally causes no overt clinical signs, it can cause profound changes in the immune system, affecting. The jackson laboratory handbook on genetically standardized mice.
More inactive and maintenance time was spent there, too. Grab the mouses neck, with fingers touching the shoulder blades. Mouse our primary serology testing method is the multiplexed fluorometric immunoassay. Twocolor, userfriendly format combined with color plate sections. This wellillustrated edition offers new and updated chapters including immunology, viruses. At the nih, the lab module is typically 3 350 mm 110 wide and 10 056 mm long 330 with an aisle width of 1 525 mm 50 between. The proinflammatory panel 1 mouse control pack consists of 3 levels of controls, each containing known concentrations of all mouse cytokines that are detected by the proinflammatory panel 1 mouse kit. One of the most timeconsuming activities in research laboratories is looking up information specific to the species or strain of animal being used. Contribution of asian mouse subspecies mus musculus. The laboratory mouse was derived from the common house mouse, mus musculus.
Genetically and genomically, the human and the mouse are very similar, with many of the diseaserelated genes nearly identical. The approximate age of mouse pups can be determined by their physical attributes during the irst two weeks of life. Affordable and search from millions of royalty free images, photos and vectors. Breeding core facility bcf laboratory animal resource center larc bcf description. Entering and exiting the gcis mouse facility entering the facility, suite, and preparing the hood 1. Malocclusion in the laboratory mouse research office. Breeding core facility bcf laboratory animal resource. This book, part of the highly successful handbook of experimental animals series, allows the user quick access to any point of interest on the mouse as an.
Malocclusion occurs in mice when the incisors overgrow because the mandibular and maxillary teeth are not normally aligned and do. The discussion features an introduction to common laboratory mouse strains, including the nude mouse, whose genetic makeup renders them both hairless and immunodeficient. This is a free sample of content from manipulating the mouse embryo. Another method which can be used firstly to assess whether housing refinements are necessary. Division of laboratory animal science, utrecht university, p. The clinical symptoms due to the virus depend upon the. Malocclusion in the laboratory mouse jax notes issue 489, spring 2003. One goal in larcs mission of supporting the ucsf research and clinical enterprise is to provide to laboratories pathogenfree mice with specific genetic backgrounds as needed for experimentation.
The laboratory mouse the common laboratory mouse mus musculus, the most commonly used animal in biomedical research, is an ideal experimental animal for several reasons. Facial expression is widely used as a measure of pain in infants. Frequency from laboratory mice and rederived as soon as rare in laboratory mice, common in wild mice. Once you are ready to submit samples, visit ltm to create your order online. Technical specifications instrumentation laboratory. For example, mhc haplotype antigens of balbc mice are h2k. Overview mouse reproduction factors affecting breeding performance data collection and good colony management. Dudek3 strains of mice that show characteristic patterns of behavior are critical for research in neurobehavioral genetics. Thermoregulation and nesting material in laboratory mice. Some commonly used laboratory mouse haplotypes and. Warm the solutions to body temperature or at least room temperature immediately prior to administration, if possible.
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